One Plate-double Nutrient Endospore Activation Method

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This paper presents a simple, swift, improved and inexpensive one plate-double nutrient endospore activation method (ODNEAM) for activation of thermophilic endospore (thermospore). Revival and cultivation of dormant bacteria experiencing un-culturable state is a difficult task. Long-term preservation at low temperature (4 °C or below) under laboratory conditions pushes most of the gram-positive bacteria to form endospores and enter dormancy. After experienced dormancy and crossed specific time (more than a month) it’s difficult for those thermospores to resurrect into a living cell. The effect of low temperature, a double quantity of nutrients and high humidity (80 %) was re-revaluated to foster germination of thermospores. Employed parameters successfully revived thermospores which experience dormancy or unable to initiate the germination process upon onset of favorable conditions. This method has crucial importance for activation of the dormant thermospore. Long-term preservation under low temperature or no sub-culturing at a fixed time interval of thermophilic bacteria may be a cause for least revival rate and the progressively diminished revival rate. The inactivation of cells after long-term preservation and probable loss to science would be avoided using this one plate-double nutrient endospore activation method. This is a tribute to Harold F. Foerster−A renowned microbiologist (1983).

BR thanks University Grants Commission, New Delhi (Government of India) for awarding postdoctoral fellowship and financial (Letter No. PDFSS-2013-14-ST-MAH-4350). The authors declare no conflict of interest. The authors acknowledge the Registrar, Swami Ramanand Teerth Marathwada University, Nanded, India (SRTMUN) and the Director, School of Life Sciences, SRTMUN, for providing the laboratory facilities.

Peer reviewed