Development of monoclonal antibody-based competitive immunoassays for the detection of picoxystrobin in cereal and oilseed flours
2012
Mercader Badia, Josep Vicent | Parra, Javier | Esteve Turrillas, Francesc A. | Agulló, Consuelo | Abad Somovilla, Antonio | Abad Fuentes, Antonio
Picoxystrobin is a new generation fungicide primarily developed to be used in cereal crops. In the present study a novel collection of specific monoclonal antibodies has been produced using different immunizing haptens based on a carboxy-functionalized identical spacer arm attached to alternative positions of the target pesticide molecule. Two competitive enzyme-linked immunosorbent assays have been developed employing hapten heterology, one using the antibody-coated direct format and the other in the conjugate-coated indirect format. Both immunoassays have been characterized in terms of selectivity, solvent tolerance, and buffer conditions, affording similar limits of detection at or below 0.1 μg/L. Finally, the optimized assays were applied to the analysis of picoxystrobin in wheat, corn, oat, barley, and soybean flours. Average recovery values from spiked samples were between 84 and 115%.
显示更多 [+] 显示较少 [-]This work was supported by Ministerio de Educación y Ciencia (AGL2006-12750-C02-01/02/ALI) and cofinanced by FEDER funds. J.P. was hired by Consejo Superior de Investigaciones Científicas (CSIC) under a predoctoral I3P contract. F.A.E.-T. and J.V.M. were also hired by CSIC with postdoctoral contracts, the former under the JAE Program and the latter under the Ramón y Cajal Program. All contracts were cofinanced by Ministerio de Ciencia e Innovación and by the European Social Fund.
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