Resistance evaluation of winter barley accessions and varieties to strains of barley yellow mosaic virus and barley mild mosaic virus under field conditions
2008
Rabenstein, Frank | Götz, Reinhard | Huth, Winfried
In extensive screening programmes genotypic differences in reactions of 82 accessions in the period 2006/2007 and 83 in 2007/2008 and 5 varieties of winter barley to the yellow mosaic virus complex were evaluated at four different locations. The fi eld trials were carried out on two sites where the soil was natural infested with both BaYMV-1 and BaMMV and at two other locations additionally infested with the resistance breaking pathotype BaYMV-2. Resistance to BaMMV and BaYMV-1 caused by rym4 was found quite frequent within the primary gene pool of Hordeum vulgaris. Sixty nine accessions tested in 2006/2007 and 74 tested in 2007/2008 exhibited no visible virus symptoms on the leaves of all inspected plants. A few number of accessions reacted inconsistently due to inhomogeneous splitting of breeding material. Among the 5 tested varieties 3 (Naomie, Jorinde and Fridericus) were resistant to BaMMV and BaYMV-1 in both evaluation periods; no virus could be detected serologically neither in tissue print immunoassay or ELISA nor by RT-PCR. In contrast, only very few accessions were unaffected by the pathotype BaYMV-2. In 2006/2007 seven breeding lines remained virus free and three other lines gave confl icting results due toa single infected plant in the plot. In 2007/2008 three of the accessions previously tested virus free did not became infected by BaYMV-2 and four other winter barley lines reacted inconsistent. The variety Jorinde carrying the resistance gene rym5 remained virus free in all examinations.
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出版者 JKI
