Direct recovery of infectious Pestivirus from a full-length RT-PCR amplicon
2008
Rasmussen, T.B. | Reimann, Ilona | Hoffmann, Bernd | Depner, Klaus Robert | Uttenthal, A. | Beer, Martin
This study describes the use of a novel and rapid long reverse transcription (RT)-PCR for the generation of infectious full-length cDNA of pestiviruses. To produce rescued viruses, full-length RT-PCR amplicons of 12.3 kb, including a T7-promotor, were transcribed directly in vitro, and the resulting RNA transcripts were electroporated into ovine cells. Infectious vir-us was obtained after one cell culture passage. The rescued viruses had a phenotype similar to the parental Border Disease virus strain. Therefore, direct generation of infectious pestiviruses from full-length RT-PCR cDNA products could be a valuable instrument for virus rescue, conservation and further characterization. (c) 2008 Elsevier B.V. All rights reserved
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书目信息
出版者 Elsevier Science
ISSN 1879-0984 | 0166-0934