Influence of cytokinin and ammonium nitrate on elongation of adventitious buds in Norway Spruce (Picea abies)
2007
Ewald, Dietrich | Jianjun, Hu
德语. The capability of different cytokinins (BA, kinetin, zeatin, 2iP) to stimulate the induction and development of adventitious buds was determined after single and repeated induction steps with hypocotyl explants. BA was the most effective cytokinin as far as it concerned the number of induced adventitious buds. Zeatin, although less effective for this pattern, promoted the elongation of buds even after repeated induction cycles contrary to BA. This is important to get propagating bud clusters with the ability to form elongating shoots. A five-year-old propagating clone line of adventitious bud clusters was used to study the influence of zeatin and ammonium nitrate on the shoot elongation. Three induction variants (6.84, 13.68, 20.52 micro-mol x L/1 zeatin, each with 0.69 micro-mol x L/1 kinetin for 4 weeks) were tested. The highest zeatin concentration induced the largest number of elongating buds. The influence of one subculture step with an enhanced ammonium nitrate concentration duri
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