Rapidin-vitroandin-vivodetection ofChalara fraxineaby means of mass spectrometric techniques
2013
Pham, Lam Huong | Zaspel, Irmtraut | Schümann, Michael | Stephanowitz, Heike | Krause, Eberhard
For the first time, mass spectrometric (MS) techniques were employed to rapidly detect the pathogen Chalara fraxinea in-vitro and directly in-vivo in tissues of diseased ash trees caused by C. fraxinea, using a range of characteristic novel secondary metabolites of C. fraxinea as chemical markers for the presence of the pathogen. We have found an evident correlation between the presence and amount of these-only for C. fraxinea characteristic and novel-secondary metabo-lites (named chalarafraxinines) and the degree of disease of respective infected ash seedlings. As demonstrated in this work, the MS based high-throughput-screening approach constitute an alternative to the time consuming and expensive micro biological isolation procedures for detection of the pathogen C. fraxinea and furthermore, can be used to rapidly test ash genotypes for resistance/susceptibility to C. fraxinea infection.
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出版者 Scientific Research Publ.
ISSN 2158-2742 | 2158-2750