Use of molecular markers associated with resistance to biotic and abiotic environmental factors in developing breeding material for tomato and pepper in Belarus

O. G. Babak; E. V. Drozd; N. A. Nekrashevich; N. V. Anisimova; K. K. Yatsevich; P. V. Shesteren; I. Е. Bayeva; N. A. Nevestenko; I. G. Puhachova; M. M. Dobrodzkin; A. V. Kilchevsky

Use of molecular markers associated with resistance to biotic and abiotic environmental factors in developing breeding material for tomato and pepper in Belarus

2025

Relevance. The development of a system of molecular markers that allows identifying the genetic determinants of resistance to pathogens, as well as the typing of alleles involved in the regulation of anthocyanin accumulation, is the most important condition for increasing the efficiency of the breeding process aimed at enhancing the resistance of cultivated crops to biotic and abiotic stresses.Methodology. The work involved molecular genetic methods of DNA isolation, PCR analysis, restriction, and evaluation of amplification and restriction products in agarose or polyacrylamide gels. The material used included the diverse collections of Solanum lycopersicum and Capsicum annuum, as well as the specimens of closely related wild species.Results. The paper evaluates the effectiveness of 25 molecular markers presented in the literature associated with resistance to tomato and pepper diseases caused by fungal, bacterial, viral pathogens, as well as nematodes. Markers to the alleles of MYB transcription factor genes associated with the regulation of anthocyanin accumulation in tomato (SlMyb12, Anthocyanin1, Anthocyanin2, An-2-like and Atroviolacium) and pepper (Myb113-like1, Myb113-like2 and ETC3-2), recommended for the breeding process aimed at increasing resistance to stressful biotic and abiotic environmental factors, are presented.

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