LUCIA To Decipher Metal Homeostasis And Toxicity In Plants
2014
Isaure, M.-P. | Huguet, Stéphanie | Grillet, Louis | Mari, Stephane | Trcera, N | Laboratoire de Chimie Analytique Bio-Inorganique et Environnement (LCABIE) ; Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS) | Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) ; Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Synchrotron SOLEIL (SSOLEIL) ; Centre National de la Recherche Scientifique (CNRS)
Some metals such as iron (Fe) are essential to plants whilst others such as cadmium (Cd) are toxic even at low concentration. The identification of the chemical forms of these metals (speciation) and their localization at the tissue and cell level are crucial to understand theirtrafficking and storage and finally the ability for the plant to cope with their potential toxicity or requirement. LUCIA beamline offers the possibility to access the metal distribution thanks to micro X-ray fluorescence with a micrometer sized lateral resolution and to investigate the metal speciation using micro X-ray Absorption Near Edge Structure. Importantly, the beamline is designed for measurements in cryogenic conditions, which is necessary to limit metal redistribution and speciation change in biologic samples. Here, we present twoexamples of our work performed on Lucia during the last years. The first one deals with Fe homeostasis and the discovery of an important pool of iron in plant cell nucleus (1) as well as the Fe species in plant embryos (2). The second example is dedicated to the study of Cd sequestration in Anthyllis vulneraria used as a pioneer plant in the phytostabilization of mining sites (3).REFERENCES1. H. Roschzttardtz, L. Grillet, M.P. Isaure, G. Conejero, R. Ortega, C. Curie, and S. Mari, J. Biol. Chem. 286, 27863-27866 (2011).2. L. Grillet, L. Ouerdane, P. Flis, M. Thi Thanh Hoang, M. P. Isaure, R. Lobinski, C. Curie, and S. Mari, J. Biol. Chem. 289, 2515-2525(2014).3. S. Huguet, S. Soussou, J. C. Cleyet-Marel, N. Trcera, M. P. Isaure, ‘Rhizostabilization of a mine tailing highly contaminated:preliminary study of Cd localization and speciation in Anthyllis vulneraria in ‘Proceedings of the 16th International Conference onHeavy Metals in the Environment’, edited by N. Pirrone, Roma, Italy, 2013, n°19008.
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