Assessment of In vitro Solubility of Stone Grit- a Major Calcium Source in Poultry Feed
2025
K. Premkumar , K. Premkumar | DURAISAMY, KANNAN | PERIYANNAN, VASANTHAKUMAR | KALIMUTHU, RAJENDRAN | SUBRAMANIAM, RAMAKRISHNAN | AMARESAN, SERMA SARAVANA PANDIAN
In-vitro solubility experiments were conducted on 156 stone grit samples to analyze the major factors influencing solubility and their strength of correlation by using five different strengths of HCl (0.1N, 0.2N, 0.3 N, 0.5 N, and 1N), three different volumes of HCl (200ml, 100ml, 50ml), two different reaction duration (10 minutes, 30 minutes), two different particle sizes (2mm, 4mm) and two different sample weights (1g, 2g) based on the weight loss method. Solubility data analyzed in multiple linear regression model revealed that the variables such as stone size, strength of HCl, volume of HCl, and reaction duration were found to be statistically significant (p<0.01) and the sample weight was statistically not significant. The strength of HCl, volume of HCl, and duration were found to be positively correlated, whereas the stone size was found to be negatively correlated with solubility. The regression equation was derived to predict the solubility values of stone grit of different particle sizes of stone grit easily with known ideal sample weight, HCl volume and strength, and reaction duration which was suggested by taking 2 grams of sample to react with 100ml of 0.2N HCl strength for 10 minutes.
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