Ecotypes of Bacillus cereus sensu lato

Zdigitalizowano i udostępniono w ramach projektu pn. Rozbudowa otwartych zasobów naukowych Repozytorium Uniwersytetu w Białymstoku – kontynuacja, dofinansowanego z programu „Społeczna odpowiedzialność nauki” Ministra Edukacji i Nauki na podstawie umowy BIBL/SP/0040/2023/01.

Bacillus cereus sensu lato is a group of bacteria common in the environment, which have an important impact on the activity of humans. Their taxonomy is still in doubt, mainly because of their properties enecoded by plasmid-borne genes that may potentially undergo horizontal gene transfer. Here we present genetic and phenotypic data that support the idea of a bacterial group (named B. cereus sensu lato) instead of several independent taxa. We conclude that B. cereus sensu lato is composed of distinct, but closely related ecotypes.

This publication was financed by the Ministry of Science and Higher Education as a part of subsidies for maintaining the research potential granted to the Faculty of Biology and Chemistry at the University of Bialystok.

M. Bartoszewicz: [email protected]

Marek Bartoszewicz - Department of Microbiology, Institute of Biology, University of Bialystok

Urszula Czyżewska - Department of Cytobiochemistry, Institute of Biology, University of Bialystok

Magdalena Siemieniuk - Department of Cytobiochemistry, Institute of Biology, University of Bialystok

Sebastian Maciak - Department of evolutionary and physiological ecology, Institute of Biology, University of Bialystok

Bartoszewicz, M. Święcicka, I., Buczek, J. (2006) Cereulidyna i enterotoksyny Bacillus cereus sensu lato. Medycyna Weterynaryjna, 62: 28-31.

Bartoszewicz, M., Bideshi, D.K., Kraszewska, A. Modzelewska, E., Swiecicka, I. (2009) Natural isolates of Bacillus thuringiensis display genetic and psychrotrophic properties characteristic of Bacillus weihenstephanensis. Journal of Applied Microbiology, 106: 1967-1975.

Bartoszewicz, M., Czyżewska, U. (2017a) Spores and vegetative cells of phenotypically and genetically diverse Bacillus cereus sensu lato are common bacteria in fresh water of northeastern Poland. Canadian Journal of Microbiology, 63: 939-950.

Bartoszewicz, M., Czyżewska, U. (2017b) Taksonomia, wirulencja i cykle życiowe Bacillus cereus sensu lato. Postępy Mikrobiologii, 56: 440-450.

Bartoszewicz, M., Marjańska, P.S. (2017) Milk-originated Bacillus cereus sensu lato strains harbouring Bacillus anthracis-like plasmids are genetically and phenotypically diverse. Food Microbiology, 67: 23-30.

Ehling-Schulz, M., Vukov, N., Schulz, A., Shaheen, R., Andersson, M., Märtlbauer, E., Scherer, S. (2005) Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus. Applied and Environmental Microbiology, 71: 105-113.

Jensen, G.B., Hansen, B.M., Eilenberg, J., Mahillon, J. (2003) The hidden lifestyles of Bacillus cereus and relatives. Environmental Microbiology, 5: 631-640.

Kaminska, P.S., Yernazarova, A., Murawska, E., Swiecicki, J., Fiedoruk, K., Bideshi, D.K., Swiecicka, I. (2014) Comparative analysis of quantitative reverse transcription real-time PCR and commercial enzyme imunoassays for detection of enterotoxigenic Bacillus thuringiensis isolates. FEMS Microbiology Letters, 357: 34-39.

Kroteń, M.A., Bartoszewicz, M., Święcicka, I. (2010) Cereulide and valinomycin, two important natural dodecadepsipeptides with ionophoretic activities. Polish Journal of Microbiology, 59: 3-10.

Lechner, S., Mayr, R., Francis, K.P., Prufß, B.M., Kaplan, T., Wießner-Gunkel, E. Steward, G.S.A.B., Scherer, S. (1998) Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group. International Journal of Systematic Bacteriology, 48: 1373-1382.

Małek, W., Wdowiak-Wróbel, S., Kalita, M., Święcicka, I. (2005) W poszukiwaniu koncepcji gatunku bakteryjnego. Postępy Mikrobiologii, 44: 323-328.

Melnick, R.L., Testen, A.L., Poleatewich, A.M., Backman, P.A., Bailey, B.A. (2012) Detection and expression of enterotoxin genes in endophytic strains of Bacillus cereus. Letters in Applied Microbiology, 54: 468-474.

Raymond, B., Bonsall, M.B. (2013) Cooperation and the evolutionary ecology of bacterial virulence: the Bacillus cereus group as a novel study system. Bioessays, 35: 706-716.

Reiter, L., Kolsto, A-B., Piehler, A.P. (2011) Reference genes for quantitative, reverse-transcription PCR in Bacillus cereus group strains throughout the bacterial life cycle. Journal of Microbiological Methods, 86: 210-217.

Swiecicka, I., Van der Auwera, G.A., Mahillon, J. (2006) Hemolytic and nonhemolytic enterotoxin genes are broadly distributed among Bacillus thuringiensis isolated from wild mammals. Microbial Ecology, 52: 544-551.

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