Estradiol-17β Hormonunun Boğa Spermlerinin Motilitesi, Canlılığı ve Akrozom Anormalliği Üzerine Etkisi
2019
ÇİFTCİ, Hüseyin Baki
The objective of this study was to measurethe effect ofadding estradiol-17β (E2) on sperm parameters in culture. This is because of the exposure of bulls to the estrogenic effect of phyto-estrogens in plants. Semen obtained from the Holstein bulls was devoted into 4 groups and cultured in medium without E2(C, Control) orcultured in medium containing 2 (T1), 4 (T2) or 8 μg(T3) E2 for 24h. The number of live, motile, sperm cells bearing damaged or lost acrosomes, sperm cells bearing cytoplasmic droplets and abnormal tails were counted at the 0th, 4th, 18th and 24thhours of incubation. Addition of 2 μg E2/mL to sperm cultureincreased the number of total motile sperm cells at 18thhours of incubation as compared to the control group. At the 4th hours of the incubation, the numbers of forward moving sperm cells were significantly higher in cultures supplemented with 2 or 8 μgE2 /mL as compared to the control groups. Supplementation of lower doses of E2 (2 or 4 μg/mL) did not influence the viability,but higher dose E2 (8 μg/mL) decreased the viability at 4th and 24th hours of incubation as compared control group. The number of sperm cells with lost acrosome at 24th hours of incubation was significantly higher in control group as compared to others.Even the data, presented here, show that the addition of E2 increase the number of motile sperm cells, but increased number of motile sperm cells is only not the parameter showing fertilizing ability of sperm. Also addition of E2 caused non-significant damages to the membrane integrity of sperm cells at 4th and 18th hours of incubation. It cannot be said that supplementation ofE2 increase the fertilizing potential of sperm cells according to the data presented here.
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