Investigating the effects of the anti-yersiniosis vaccine using the bath method on some biochemical parameters of the blood serum of Huso huso

Yersiniosis, caused by Yersinia ruckeri, is recognized as one of the most significant diseases affecting cold-water and sturgeon fish in aquaculture. Vaccination has emerged as the most cost-effective and sustainable method for controlling infectious diseases in fish. This study investigated the effects of a bath vaccine against yersiniosis on certain biochemical parameters in the serum of beluga sturgeon (Huso huso). For this purpose, 400 to 500 juvenile fish with an average weight of approximately 10 gr were divided into 14 tanks and raised for 10 to 14 days to acclimatize to environmental conditions. The treatments included: A single bath vaccination with anti-yersiniosis vaccine (with Brand name: Antiyersin) for 10 gr fish, two-stage bath vaccination against yersiniosis for 10 gr fish. two-stage hyperosmotic bath vaccination against yersiniosis (antiyersin) for 10 gr fish. Single injection vaccination against yersiniosis for 10 gr fish and a control group of unvaccinated fish. The impact of the vaccine on the biochemical factors in the blood of beluga sturgeon was also evaluated. The results indicated that in the vaccinated treatments, biochemical blood indices such as glucose, uric acid, urea, cholesterol, total protein, triglycerides, calcium, creatinine, and albumin were measured. The findings from this study demonstrated that the vaccine had a better effect on the health of beluga sturgeon weighing between 10 to 15 gr, with significant differences observed in glucose, uric nitrogen, uric acid, triglycerides, total cholesterol, and calcium levels between the vaccinated groups and the control group. However, there was no significant effect on total protein, creatinine levels, and the gene expression of dismutase among the vaccinated groups compared to the control group.