Molecular Detection of Trypanosomatids in Rodents and Marsupials in the State of Amapá, Brazil
2025
Lourdes Marina Bezerra Pessoa | Claudia Regina Silva | Kamila Gaudêncio da Silva Sales | Darlison Chagas de Souza | Lucas Lisboa Nunes Bonifácio | Rafaela Lira Nogueira de Luna | Filipe Dantas-Torres | Lúcio André Viana
Trypanosomatids of the genera <i>Trypanosoma</i> and <i>Leishmania</i> are parasites of medical and veterinary importance that infect mammals, including humans and domestic and wild animals. Among mammals, rodents and marsupials play a crucial role in maintaining and spreading the zoonotic transmission cycle of these parasites. The present study aimed to detect the natural occurrence of <i>Trypanosoma</i> spp. and <i>Leishmania</i> spp. in rodents and marsupials in the state of Amapá, northern Brazil. In total, 137 samples were analyzed, of which 19 (6 marsupials and 13 rodents) were positive for trypanosomatid DNA. Partial sequences of the 18S rRNA gene of trypanosomatids were obtained from 10 out of 19 positive samples. Specifically, an undescribed <i>Trypanosoma</i> sp. was detected in <i>Marmosa demerarae</i>, <i>Marmosa murina</i>, <i>Zygodontomys brevicauda,</i> and <i>Neacomys paracou</i>. <i>Trypanosoma cruzi</i> was detected in a <i>Philander opossum</i>, whereas sequences close to <i>Trypanosoma wauwau</i> and <i>Trypanosoma freitasi</i> were obtained from <i>Didelphis imperfecta</i> and <i>N. paracou</i>, respectively. Finally, <i>Leishmania</i> (<i>Viannia</i>) sp. was detected in <i>Mesomys hispidus</i>, <i>Hylaeamys megacephalus</i>, and <i>Z. brevicauda</i>. The present study expands the knowledge about marsupials and rodents as hosts of trypanosomatids and emphasizes the need for further studies on the role of these animals as potential reservoirs of these parasites in the Amazon region.
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