Genetic Diversity of <i>Calligonum</i> L. Species Analyzed Using Newly Developed Genomic Simple Sequence Repeats and Their Application to Conservation
2025
Jiayi Su | Xuerong Li | Shiqing Liu | Xiyong Wang | Daoyuan Zhang | Borong Pan | Mengmeng Wei | Jancheng Wang | Wei Shi
Various species from the genus <i>Calligonum</i> L. are widely used in erosion control in Northwest China. Their widespread use and breeding efforts highlight the need for the conservation of the high-quality germplasm resources of this genus. To assess genetic diversity, the genome of <i>Calligonum junceum</i> was screened, and 380,328 SSR loci were identified. After further screening, 17 microsatellite markers were identified and transferred to 11 other species of the genus. When analyzed together, the 11 species showed significant discrimination power (PIC = 0.623), and we detected that the mean number of alleles per locus was 2.684, the overall heterozygosity observed value was 0.295, and the expected heterozygosity (<i>He</i>) was 0.373. Genetic diversity in <i>Calligonum mongolicum</i> was the highest at <i>I</i> = 1.305, <i>He</i> = 0.647, and that of <i>Calligonum gobicum</i> was the lowest at <i>I</i> = 0.127, <i>He</i> = 0.072. The results of this study provide reference SSR primers for the genus <i>Calligonum</i>, supporting the improvement of its germplasm conservation efforts.
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