Enzymatic hydrolysis of cottonseed protein enhances appetite and intestinal health of Litopenaeus vannamei
2025
Jili Wang | Dawei Zhao | Yujia Yang | Tianbi Li | Shipin Wang | Shaoying Lv | Beiping Tan | Shuyan Chi
This study examined the effects of enzymatic hydrolysis cottonseed protein (ECP) on the appetite and intestinal health of Litopenaeus vannamei (0.16 ± 0.01 g). Based on the control diet (fishmeal 18 %, crude protein 40 %, crude fat 7.5 %), 2 %, 4 %, 6 %, 8 % and 10 % of ECP were added to replace fishmeal in equal amounts to formulate six isonitrogenous and iso-lipidic experimental diets named ECP0 (Control), ECP2, ECP4, ECP6, ECP8, and ECP10, respectively. After an 8-week feeding trial, the serum orexin levels were significantly increased (P < 0.05); and leptin, peptide YY and cholecystokinin were significantly reduced when shrimp ingested diet contained ECP， as well as their genes expression levels (P < 0.05). Compared with the ECP0 group, the weight gain rate of the shrimps in group ECP2 had significantly increased (P < 0.05). Besides, intestinal fold height and muscular thickness of shrimps in group ECP2 were markedly increased, and up-regulated genes expression levels for periplasmic membrane factors (P < 0.05). Significantly increased intestinal amylase, lipase, trypsin and chymotrypsin activities were found in shrimps fed diet ECP2 and ECP4 (P < 0.05). The addition of ECP significantly decreased Vibrio, Photobacterium and Tenacibaculum abundance, and increased Ruegeria and Marivita abundance in shrimp intestine (P < 0.05), especially the supplemented 2 % ECP significantly improved the membrane transport and amino acid metabolism functions of the intestinal flora in shrimps (P < 0.05). Therefore, the addition of 2–4 % ECP by replacing fishmeal at an equal level could enhance growth by improving appetite, intestinal morphology and the composition of gut microbiota for L.vannamei.
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