Optimizing Cryopreservation of <i>Pseudoplatystoma magdaleniatum</i> Semen: Evaluation of Two Permeable and Two Non-Permeable Cryoprotectants
2025
César Montes-Petro | Betty Rodríguez-Peroza | Diana Madariaga-Mendoza | Carlos Tapia-Pacheco | José Espinosa-Araujo | Víctor Atencio-García
The study aimed to evaluate the effectiveness of the cryopreservation protocols for <i>Pseudoplatystoma magdaleniatum</i> semen using dimethyl sulfoxide (DMSO) or methanol (MET) as permeable cryoprotectants at two concentrations (5% and 10%) combined with 12% egg yolk (Y12%) or 5% skimmed milk powder (SMP5%) and glucose (6%), resulting in eight treatments. A semen pool (<i>n</i> = 8) was diluted in a 1:4 ratio, packed in 2.5 mL straws, and frozen in nitrogen vapors. It was thawed at 35 °C for 90 s. Sperm kinetics and motility duration of fresh, prefrozen, and thawed semen were analyzed using a CASA system. The osmolarity of seminal plasma and cryosolutions was estimated. Fertilization (F) and embryo viability (E) rates of thawed semen were evaluated. The osmolarity of seminal plasma was 251.1 ± 3.3 mOsmol/kg and, in the cryosolutions, ranged between 1248.3 ± 19.9 mOsmol/kg (DMSO5% + Y12%) and 3488.2 ± 1.5 mOsmol/kg (MET10% + Y12%). After thawing, total motility ranged from 38.2% to 60.5%, representing a significant reduction compared to fresh semen (95.4 ± 2.1%) (<i>p</i> < 0.05). The best fertilization and embryo viability rates of thawed semen were obtained with DMSO5% + SMP 5% (F = 20.7%, E = 11.7%) and MET10% + SMP5% (F = 20.1%, E = 11.5%) (<i>p</i> < 0.05). A cryopreservation protocol for <i>P. magdaleniatum</i> semen with 5%DMSO or 10%MET combined with SMP5% is possible, but further study is necessary to optimize its fertilizing capacity.
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