Multi-drug resistant pattern of Escherichia coli isolated from cattle, human diarrheal cases and environment samples in Basrah Province
2025
Mariam Issam Abdullah, Rasha M. Othman, Nawres Norri Jaber.
The aim of the present study is to diagnose the multidrug resistant Escherichia coli that has been isolated from human and animal’s diarrheal cases and from environmental samples, the results of bacterial culturing methods revealed that out of 250 collected samples, 53 of isolates exhibited characteristic greenish metallic sheen colonies on EMB agar with bright pink lactose fermenting capabilities on MacConkey agar. Additionally, the conventional PCR assay was used to identified the E. coli species based on uidA gene, the results shown that all of isolates (100%) having the target gene. The results were also revealed the incidence rate of MDR Escherichia coli isolated among cattle, humans, and the environment which seem to have a similar rates of resistance for the five classes of antibiotics. Furthermore, MDR E. coli isolated from humans, animals, and the environment exhibited a high 100% resistance to amoxicillin-clavulanic acid, as well as (66.66, 83.33, and 72.72) resistance to aminoglycosides (gentamicin and streptomycin), 56.66%, 66.66%, 81.81% resistance to tetracycline (tetracycline), and 66.66%, 50%,100% resistance to dihydrofolate (trimethoprim), while it showed less resistance to quinolones (Nalidixic acid) 20%, 25%, and 18.18% respectively.
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