GENOTOXICITY OF DIAZINON IN MALE ALBINO RATS FED ON DIET SUPPLEMENT WITH CHITOSAN
2014
Mohammed J. Alwan | Jihad A. Ahmed
In order to determine the genotoxic effects of diazinon and the role of chitosan to neutralize these effects, our study performed in (24) male rats (Rattus norvegicus) were divided into four groups and treated for (60) days as following, group (A) treated with normal saline and served as control, group (B) treated with [(1/10LD50) 3.8mg/kg. bw] of diazinon, group (C) treated with [(1/10LD50) 3.8mg/kg. bw] of diazinon and fed on diet supplement containing (1gram/1kg ration) chitosan, group (D) fed on diet supplement containing (1gram/1kg ration) chitosan only. The genotoxic effect of diazinon was evaluated by using the micronucleus assay showed increasing of micronucleated polychromatic erythrocytes were (11.6%) in group B, while (7%) in group C . The chromosomal aberration showed increase of presence of chromosomal aberration in group B was (7.5±1.04), while in the group C showed mild elevation in (3.25±0.8). The polymorphism of GSTM1 and GSTT1 genes showed highly incidence of both genes polymorphism in group B was (66.6%) while group C was (50%) . we concluded that diazinon is genotoxic pesticide and chitosan ameliorate it effects.
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