PCR BASED DETECTION OF GRAM NEGATIVE PSYCHROTROPHIC BACTERIA IN COWS RAW MILK
2016
Amjed A. Alrazakkazal | Fawziah A. Abdullah
Investigations of the proteolytic Gram Negative Psychrotrophs(GNP) bacteria was the basal objective of this study. A conventional diagnostic PCR technique based on three pairs of primers including SerAgeneto amplify an 950 bp fragment of Acinetobacter sppDNA,serAgene for/A. hydrophila ( 650bp): and aprgene for/S. marcescens (500bps) was done.In the present study the 29 bacterial isolates obtained from 100 cows raw milk samples were collected randomly from healthy cows with different age and breed present in different farms of Thi-Qar province, previously refrigerated for 72 hr. These isolates subjected to cultural-based enrichment and PCR- based identification .The present results revealed that the raw milk GNP contamination overall ratio was 29% . Acinetobacter spp were the most predominant bacteria (16%) among the studied GNP contaminants, while A.hydrophila appeared in a ratio of 7% and S. marcescens showed the lower ratio ( 6%). ,the results of the studied genes product of GNP bacteria was considered to be highly statistically significant (P>0.001).The distribution of studied GNP according to age ,parturition number and breed of studied animals was investigated. The effect of these factors on the PCR-based identification results was considered to be not statistically significant(P>0.05) however, the higher overall ratio(29.1%) for cow raw milk contamination was observed in raw milk of cows between
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