EVALUATION OF SEMEN SEX RATIO IN COOLED AND FROZEN SEMEN STRAWS BY REAL-TIME PCR
2016
Baqer Ja’fer. Hassan | Souhayla Oneeis Hussain | Mohammed Sh. Jebur
The study was demonstrated on quantitative evaluation of SRY and ZFX gene of (120) semenstraws from 10 Holliston bull (60) straws in cooling state 5C° and 60 straws after deep freezingin liquid nitrogen) . The samples collected from the Artificial Insemination Center of Abo-Ghreeb / Baghdad . All samples were sent to the laboratory for DNA extraction using (QiampDNA extraction Kit) and primer design then testing in real time PCR. The results showed therewere highly significant variation in the sex ratio of cooled and frozen semen straws which variedbetween (35%-59% in ZFX , 37%-58% in SRY and 40%-69% in ZFX , 30%-53% in SRY forfrozen and cooled semen respectively ) . The study conclude that it was easy and possible todetect the quantity of sex ratio for each bull through using real time PCR . The freezing processof semen could cause decrease in the percentages of SRY (minimum 30%) and increase in thepercentage of ZFX (maximum 69%) .
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