CLINICAL ,HEMATOLOGICAL AND DIAGNOSTIC STUDIES OF MYCOPLASMA WENYONII INFECTION IN CATTLE OF BASRAH GOVERNORATE BASRAH ,IRAQ

The present work were conducted on (225) local cattle breeds of both sexes , and ofdifferent ages in Basarh governorate (Basrah –Iraq). Two hundred local cattle breedswere naturally infected with. Mycoplasma wenyonii and (25) clinically normal cattlebreeds served as controls. According to age, diseased animals were divided into fourage groups (50) animals for each. Animals were found clinically infectedwith Mycoplasma wenyonii which diagnosed based on Giemsa stain blood smears andconfirmed with PCR test technique. Diseased cattle show sings of partial or completeloss of appetite ,anemia with pale and / or icteric mucous membranes , decrease milkproduction , rapid and difficult respiration, enlargement of superficial lymph nodes ,rough coat , lethargy , weight loss and edema of lower hind limbs, In addition , bodytemperature ,respiratory rate ,heart rate and capillary refilling time were increasedstatistically , compared with controls ,Furthermore statistically significant decrease(P<0.05) were encountered in ruminal contractions .Results were also indicated that TRBCs, Hb, and PCV values of diseased cattle weresignificantly decrease than controls thereby macrocytic hypochromic type of anemiawas indicated. Results were also shown a significant increase in TLC as a result ofsignificant increase lymphocytes, Mycoplasma wenyonii appear coccoid or rod shape,structures, However it might found individually or in chains on the erythrocyte cellwall, Moreover diagnosis were confirmed by PCR,Since out of (96) blood samples(80) (83.3%) were found positive. Results were also revealed that animals of 2-3years old were highly infected compared with other age groups. Changes of bloodclotting factor indices were also noticed in diseased cattle compared with controls andthe results showed significant decrease (P<0.05) in the mean values of total thrombocytes count and Fibrinogen time ,whereas significant increase (P<0.05) weredetected in thrombocytes volume, thrombocytes distribution width, prothrombin time, clotting time, and activated partial thromboplastin time. It have been concluded thatMycoplasma wenyonii infected local cattle breeds of Basrah Governorates and lead tosubstantial effect including decrease milk yield and anemia's ,which might terminatedwith economic losses ,Therefore all cattle reared in this area must be screened.