PURIFICATION OF LACTATE DEHYDROGENASE-C4 FROM SEMINAL PLASMA OF MALE INFERTILITY
2018
Shihab A. Al-Bajari | Nashwan I. Al-Lehebe | Sarmad A. Hazzaa
Male factor infertility contributes partially and solely to the problem of childlessness inaround 50% of the cases. Unfortunately, 30 -- 50% of the etiologies of male infertility are unknownand therefore, no specific therapy can be instituted. Sperm count and sperm motility are primeparameters that determine the functional ability of spermatozoa.This study included determinationof lactate dehydrogenase-C(LDH) activity in male infertility (n=23)and its relationship with thedifferent parameters of the seminogram. Also deals with partial purification lactatedehydrogenasefrom semen plasma of male infertility patients by dialysis, and ion exchange DEAEcellulosetechniques. There was statistically significant correlation was found between LDH activityand Sperm Count , morphology % and Motility % (r=0.486), (r=0.263)and (r=0.444) in fertile grouprespectively, also that the correlation in infertile group was statistically significant between LDHactivity and Sperm Count , morphology % and Motility % (r=0.431), (r=0.671)and (r=0.336)respectively.Oneproteinous peak of lactate dehydrogenaseactivity is obtained with specific activity of 0.861u\mgprotein and purification fold 6.10 compared to crude enzyme. The kinetic characteristics of partiallypurified lactate dehydrogenaseare studied. The maximum activity is obtained at 250μg of enzyme,Tris-Hcl buffersolution at pH 7.9, temperature 37ᵒC, incubation time 5 min., with 10mMK+, Vmax and Km values of partially purified lactate dehydrogenase1.176 unit/ml and 27.02 mM with thesubstrate sodium pyruvateare found respectively
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