MOLECULAR DETECTION OF METHICILLIN-RESISTANT Staphylococcus aureus ISOLATED FROM MILK AND CHEESE OF COW AND BUFFALOES IN BASRAH CITY
2018
Weam Abd Ali Aboud | Bassam Yasein Khudaier
In the present study, 135 samples were collected from different animal's including:75 samples were from milk and 60 samples were from cheese, 54 (40%) sample were foundto be harbored with Staphylococcus aureus. The rate of S. aureus isolates was 50% inbuffalo's cheese, 40.54% in buffalo's milk, 36.8% in cow's milk, and 33.33% in cow'scheese. 100% strains were Methicillin Resistance Staphylococcus aureus. The antibioticsensitivity test was determined against 8 common antibiotics by the agar disc diffusionmethod on Muller-Hinton agar. These antibiotics were amoxicillin (25mcg), ampicillin(10mcg), Oxacillin (1mcg), chloramphenicol (30mcg), erythromycin (15mcg), gentamycin(10mcg), methicillin (5mcg), and tetracycline (30mcg). S. aureus strains were screened byPCR for 16S rRNA and nuc genes. 49 out of 54 S. aureus isolates were yielded productswith molecular weight approximately (228 bp) corresponding to 16S rRNA gene, 42 out of54 isolates were give products with molecular weight approximately (270bp) correspondingto nuc gene, 22 and 4 out of 30 S. aureus isolates were give products with molecular weightapproximately (310bp and 509bp) corresponding to mecA and femA genes, respectively.
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