MOLECULAR CHARACTERIZATION OF Parabronema skrjabini IN BASRAH PROVINCE, IRAQ
2018
Sajjad S. Soud | Ghazi Y. Azzal | Suzan A. Al-Azizz
Parabronema skrjabini is a spirurid nematode of the family Habronematidae thatlives in the abomasum of ruminants such as sheep and goats. The purpose of this studywas to investigate the molecular aspects of Parabronema skrjabini in Basrah. Theworms were collected from slaughtered sheep and goats in Basrah slaughter housebetween the period from June, 2016 to January, 2017, with total number of abomasum'sexamined sheep (576) and goat ( 150 ). An internal transcribed spacer 2 ribosomalDNA (ITS2-rDNA) fragment of P. skrjabini was amplified by polymerase chainreaction (PCR) using a pair of specific primers (Para-Ir-R and Para-Ir-F). ITS2-rDNAsequences by using PCR technique assay which based on a 783-bp long sequence ofthe 28S rRNA gene, the total genomic DNA has been extracted by extracting kit withsome modification. ITS2 homology in different isolates was between (81–86%)compared with the sequence data in GenBank. To our knowledge, this is the first studyin Iraq exploring the genetic diversity of Parabronema in sheep and goats.
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