Comparative study of the glucose and trehalose addition on the extenders for goat sperm liquid storage.

Md. Emtiaj Alam; Most. Shorifa Yeasmin; Dipak Kumar Das; Md. Shohidul Islam; Soshe Ahmed; Md. Hamidul Islam; Md. Akhtarul Islam; Md. Mostofa Kamal; Aurangazeb Kabir; Alam Khan; Md. Hakimul Haque; Md. Abdul Masum; Moizur Rahman; Mst. Ishrat Zerin Moni.

Comparative study of the glucose and trehalose addition on the extenders for goat sperm liquid storage.

2025

Objectives: The objective of this study was to assess the effects of varying glucose and trehalose concentrations on tris-citric acid-egg yolk-fructose (TCEF) diluents for the short-term cold storage of goat semen. Materials and Methods: The semen sample was collected, unwashed, and divided into the following groups: control (TCEF without glucose and trehalose), TCEF + glucose (75, 150 mm), and TCEF + trehalose (75, 150 mm). Each experimental sperm group (sperm concentration: 9×107/ ml) was kept at 4°C in a refrigerator. The impact of varying glucose and trehalose levels on the quality of the spermatozoa was assessed at different time points: after dilution and at 5, 24, 48, and 72 h of refrigeration. Results: After dilution, progressive motility (PM), total motility (TM), sperm viability (SV), functional integrity (FI), and acrosome integrity of G-75, G-150, T-75, and T-150 did not differ significantly from the control. The PM, TM, SV, FI, and acrosome integrity of sperm of T-150 were considerably lower than the control, G-75, G-150, and T-75 after 5 and 24 h of cool storage. The T-75 group showed superior PM, TM, and FI after 48 h of cool storage, with noticeably greater values than the other groups. Conclusion: This study indicates that trehalose is a more favorable sugar than glucose for 48 h cool storage of buck semen, providing greater advantages in PM, TM, and PMI. [J Adv Vet Anim Res 2025; 12(1.000): 287-296]

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