The inability to induce rooting of in vitro-established Protea cynaroides microshoots has prevented the production of complete plantlets. A successful shoot-tip micrografting technique was developed using in vitro-germinated P. cynaroides seedlings as rootstocks and axenic microshoots established from pot plants as microscions. Thirty-day old seedlings, germinated on growth-regulator-free, half-strength Murashige and Skoog medium, were decapitated and a vertical incision made from the top end. The bottom ends of microshoots established on modified Murashige and Skoog medium were cut into a wedge (‘V’) shape, and placed into the incision. The micrografted explants were cultured in a growth chamber with the temperature adjusted to 25 ± 2°C, with a 12-h photoperiod. Best results were obtained by placing the microscions directly onto the rootstock without any pre-treatments. Dipping the explants in anti-oxidant solution or placing a layer of medium around the graft area led to the blackening of the microscion. Abbreviations EDTA Ethylenediaminetetraacetate - BAP 6-Benzylaminopurine - GA3 Gibberellic acid - PAR Photosynthetic active radiation
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AGRIS - 国际农业科技情报系统
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King protea
语言
英语
许可
Springer. The original publication is available at www.springerlink.com
ISSN
0167-6857, 1573-5044
类型
Journal Article; Journal Part
2025-05-29
2026-02-03
MODS