The <i>PIN</i> Gene Family in Cucumber (<i>Cucumis sativus</i> L.): Genome-Wide Identification and Gene Expression Analysis in Phytohormone and Abiotic Stress Response
2025
Yongxue Zhang | Kaili Zhu | Weiyao Shen | Jiawei Cui | Chen Miao | Panling Lu | Shaofang Wu | Cuifang Zhu | Haijun Jin | Hongmei Zhang | Liying Chang | Xiaotao Ding
The auxin efflux transporter PIN protein plays a crucial role in the asymmetric distribution of auxin on the plasma membrane, influencing the growth and development of plant organs. In this study, we identified nine members of the <i>PIN</i> gene family in the cucumber genome, which could be classified into five phylogenetic groups. These genes have diverse structures but conserved transmembrane domains. Analysis of cis-acting elements in the promoters revealed that <i>CsPINs</i> contain 48 types of cis-acting elements, predominantly light-responsive elements and plant hormone response elements. In addition, PIN proteins may interact with a variety of auxin-related proteins (including auxin response factor, auxin binding protein, mitogen-activated protein kinase PINOID, etc.) to jointly regulate the auxin synthesis and metabolic pathways. We analyzed the expression profiles of <i>PIN</i> genes in 23 tissues of cucumber using the CuGenDB database, and further investigated the expression levels of <i>PIN</i> genes in leaves and roots in response to different abiotic stresses and hormone treatments by qRT-PCR. This study provides a theoretical basis for clarifying the regulatory mechanism of the cucumber <i>PIN</i> gene family during environmental stress processes.
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