Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L. Wilczek)
2014
Sehrawat, Nirmala | Bhat, Kangila V. | Kaga, Akito | Tomooka, Norihiko | Yadav, Mukesh | Jaiwal, Pawan K.
Thirty eight novel microsatellite markers (SSRs) specific to candidate genes involved in salt tolerance were developed for detection of genetic variations in 12 mungbean genotypes variably adapted to salt stress. A 100 out of 124 putative alleles were found polymorphic between wild and cultivated genotypes (inter-specific, 80.65%), 65 were within cultivars of mungbean (intra-specific, 52.42%) and 52 were within wild genotypes (inter-specific, 41.94%). The polymorphism varied from 86.84% to 100%, while the number of polymorphic alleles ranged from 1 to 4 with an average value of 2.63 per locus. The polymorphism information content (PIC) values ranged from 0.326 to 0.875 with an average value of 0.671, which shows their effectiveness in genetic analysis. Cluster analysis resulted in the distribution of salt tolerant and susceptible genotypes in separate groups which revealed the presence of inherent variations among mungbean cultivars. These variations were explored effectively for SSR markers studies. The developed SSR markers may help along with already available markers to execute further research on mungbean. The markers may be coupled with specific loci linked with salt tolerance. The developed markers will help to identify the QTLs (quantitative trait loci) or other important genes. These markers can also be utilized for testing the purity of hybrids or diversity assessment of Vigna species for important agronomic traits.
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