DISTINCT MUSCLE STEM CELL FATES GOVERNING HYPERPLASIA AND HYPERTROPHY MUSCLE GROWTH IN FISH
2025
Jagot, Sabrina | Sabin, Nathalie | Babarit, Candice | Bugeon, Jérôme | Ralliere, Cécile | Le Grand, Fabien | Rouger, Karl | Gabillard, Jean-Charles | Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Laboratoire de Physiologie et Génomique des Poissons = Fish Physiology and Genomics Institute (LPGP) ; Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Mécanismes en sciences intégratives du vivant (MeLiS) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) | SFBD | ANR-20-CE20-0013,FishMuSC,Dynamique cellulaire et moléculaire des cellules souches musculaires lors du déclin de l'hyperplasie musculaire chez la truite(2020)
International audience
显示更多 [+] 显示较少 [-]英语. Skeletal muscle is the largest tissue in vertebrates. After birth in mammals, muscle growth is mainly driven by hypertrophy. Here, we studied Oncorhynchus mykiss which employs a unique hyperplastic growth modality. We assembled a single-cell atlas of muscle-derived cells (MDCs) across five growth stages in juvenile rainbow trout. Among the fifteen distinct populations residing in growing muscles, we identified eight myogenic subpopulations spanning quiescent, proliferative, and differentiating states. Using RNA velocity analysis, we found two myogenic trajectories, one of these waning as hyperplastic growth declines. Comparative analyses with human atlases established similarities in myogenic states between species, depending on developmental or adult stage. Then, extracellular matrix remodeling by muscle stem cell–intrinsic and extrinsic factors accompanied hyperplasia decline. Notably, we identified a tissue-resident pax7+/pdgfrα+ cell population, indicating muscle stem cells plasticity toward the fibroblastic lineage. This hyperplastic atlas in fish provides insights into mechanisms underpinning muscle growth and can be exploited for comparative studies on myogenesis across species.
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