Proteo-biochemical characterization of ovarian follicular cysts in buffaloes (Bubalus bubalis): Proteo-biochemical characterization of ovarian follicular cysts in buffaloes (Bubalus bubalis)
2022
Satheshkumar, S | Brindha, K | Srinivasa Prasad, Ch | Sakthivel, S | Namitha, B | Meenambigai, T V
Follicular cyst is one of the major factors affecting the fertility of buffaloes. The present study was conducted to assess the proteo- biochemical characters of the follicular fluid (FF) and granulosa cells (GCs) of normal and cystic follicles of buffaloes. Ovaries were collected from slaughtered buffaloes and follicles were classified based on their diameter as: small (SF: < 4mm), medium (MF: 4 – 9 mm), large (LF: > 9 mm) and cystic (CyF: > 17mm). FF and GC were collected aseptically and subjected for further studies. Biochemical analysis of FF revealed significantly high concentrations of glucose and triglyceride in CyF when compared to LF, which could be due to poor metabolic activities of the damaged follicular cells. Protein fraction analysis of the FF and GC revealed increased expression of proteins in the molecular weight ranges of around 30,70 and 200 kDa relating to insulin like growth factor binding protein, heat shock protein 70 and vasculo endothelial growth factor respectively in CyF. It was concluded that poor utilization of proteins and deranged metabolic and angiogenic proteins in the follicular microenvironment would have led to the anovulatory status and persistence as cyst in buffaloes.
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