Optimization of Proper Sterilization and Tissue Culture Conditions in Liquidambar orientalis
2024
Kaya, Ilker | Ugur, Senem | Yalcin Mendi, Yesim
Anatolian Sweetgum (Liquidambar orientalis) is an endemic sweetgum species native to Southwest Anatolia. In addition to its general use as a landscape plant, it holds significant importance due to the resin it contains. Beyond traditional propagation methods such as seed and cutting, research is being conducted on the use of tissue culture to produce more efficient and higherquality material through rapid and clonal multiplication of the sweetgum plant. In this study, we aimed to determine the appropriate sterilization method and suitable growth medium for Liquidambar orientalis. Various combinations of HgCl2 (0.1%), NaClO (20%), and fungicide (Captan, 0.3%) solutions with different durations and concentrations were tested for sterilization. The highest sterilization rate (97%) was achieved with a combination of 48 hours of fungicide (Captan, 0.3%) + 10 minutes of HgCl2 (0.1%) + 20 minutes of NaClO (20%). To investigate the effects of Thidiazuron (TDZ) and Naphthalene Acetic Acid (NAA) on plant growth, different combinations of TDZ and NAA were tested, and the best growth was observed in WPM medium containing 1 mg.l-1 TDZ and 0.1 mg.l-1 NAA.
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