Isolated microspore culture in Brassica breeding: The technique, progress, and future perspectives
2025
Ahadi, Mesbahuddin | Arabzai, Mohammad Gul
The method of isolated microspore culture is extensively employed in the generation of haploid and double haploid plants, facilitating genetic and biotechnological research while expediting breeding operations. Isolated microspore culture is superior than anther culture due to the propensity of anther walls to generate unwanted diploid and somatic calli in plants during the process. This paper encompasses an examination of doubled haploid plant development utilizing isolated microspore culture techniques in modern Brassica species. The review provides a systematic account of the Brassica genus haploid/doubled haploid plant production process, encompassing donor plant management, floral collection, microspore separation, culture initiation, embryo generation, chromosome duplication, and the subsequent development of haploid/doubled haploid plants. Thereafter, the recent advances in this technique will be surveyed, and the major obstacles facing its application in Brassica breeding programs will be discussed.
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