Investigating miR-30a′s tumor suppressor role and its targets (MYBL2, TWF1, GALNT7, PFN2) as prognostic biomarkers in lung adenocarcinoma
2025
Yunus Sahin | Zekiye Altan | Khandakar A.S.M. Saadat | Masa-Aki Ikeda | Ahmet Arslan
Lung adenocarcinoma (LUAD), the most prevalent subtype of non-small cell lung cancer, is a significant cause of cancer-related mortality due to late-stage diagnoses and limited treatment options. Identifying effective biomarkers is crucial for improving prognosis and therapeutic strategies. miR-30a-5p has emerged as a potential tumor suppressor in LUAD, yet its exact role remains unclear. In this study, we analyzed three miRNA expression datasets from the GEO database and confirmed miR-30a downregulation in LUAD through TCGA and qRT-PCR validation. We identified MYBL2, TWF1, GALNT7, and PFN2 as oncogenic targets of miR-30a by integrating miRNA target predictions with gene expression data. Expression and survival analyses revealed an inverse relationship between miR-30a and these targets, with high expression levels correlating with poor patient outcomes. Functional enrichment analysis highlighted pathways in cell cycle regulation and actin cytoskeleton organization. Our findings underscore the significance of the miR-30a regulatory network in LUAD progression, positioning miR-30a and its targets as promising prognostic biomarkers and therapeutic targets.
显示更多 [+] 显示较少 [-]