Impact of nutrient additions on free-living nitrogen fixation in litter and soil of two French-Guianese lowland tropical forests
2021
Van Langenhove, Leandro | Depaepe, Thomas | Verryckt, Lore T. | Vallicrosa, Helena | Fuchslueger, Lucia | Lugli, Laynara F. | Bréchet, Laëtitia | Ogaya, Romà | LLusià, Joan | Urbina, Ifigenia | Gargallo‐Garriga, Albert | Grau, Oriol | Richter, Andreas | Peñuelas, Josep | Van Der Straeten, Dominique | Janssens, Ivan A.
In tropical forests, free-living Biological nitrogen (N) fixation (BNF) in soil and litter tends to decrease when substrate N concentrations increase, whereas increasing phosphorus (P) and molybdenum (Mo) soil and litter concentrations have been shown to stimulate free-living BNF rates. Yet, very few studies explored the effects of adding N, P, and Mo together in a single large-scale fertilization experiment, which would teach us which of these elements constrain or limit BNF activities. At two distinct forest sites in French Guiana, we performed a 3-year in situ nutrient addition study to explore the effects of N, P, and Mo additions on leaf litter and soil BNF. Additionally, we conducted a short-term laboratory study with the same nutrient addition treatments (+N, +N+P, +P, +Mo, and +P+Mo). We found that N additions alone suppressed litter free-living BNF in the field, but not in the short-term laboratory study, while litter free-living BNF remained unchanged in response to N+P additions. Additionally, we found that P and P+Mo additions stimulated BNF in leaf litter, both in the field and in the lab, while Mo alone yielded no changes. Soil BNF increased with P and P+Mo additions in only one of the field sites, while in the other site soil BNF increased with Mo and P+Mo additions. We concluded that increased substrate N concentrations suppress BNF. Moreover, both P and Mo have the potential to limit free-living BNF in these tropical forests, but the balance between P versus Mo limitation is determined by site-specific characteristics of nutrient supply and demand.
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