Genotypic data on reference set for fodder quality in Sorghum under drought
Deshpande, Santosh | Somegowda, Kanuganahalli Vinutha | Naravula, Jalaja | Rathore, Abhishek | Jones, Chris | Deshpande, Santosh
Genotypic data on 129 reference entries of Sorghum for GWAS analysis and identification of genomic regions for fodder quality. The genotypes were sown and DNA was isolated from leaves of each accession at 4 to 6 leaf stage using the modified hexadecyltrimethylammonium bromide (CTAB) protocol from 12-day-old seedlings and genotyping done following the GBS (Genotyping by Sequencing) approach with ApeKI restriction enzyme used for complexity reduction. SNPs were called using TASSEL v5.2 GBS pipeline against Sorghum assembly v3.1 with default parameters. The raw SNP data has 129 taxa with 5176699 numbers of sites. The filtering was done with site coverage of 80%, minimum allelic frequency: 0.01, heterozygosity: 50% and a final SNP count of 11472.
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