The Influence of Phytohormones on the Efficiency of Callus Formation, Its Morphologically Properties and Content of Bioactive Compounds in In Vitro Cultures of Daucus carota L.
2022
Anna Jadwiga Keutgen | Magdalena Tomaszewska-Sowa | Aleksander Bomberski | Norbert Keutgen
The significance of cultivar colour (orange and yellow), the application of MgO during field cultivation and chosen phytohormones in the callus cultivation medium are investigated in the present study, with respect to the antioxidative properties of the obtained callus. Callus cultivation are examined as an alternative method for the production of plant antioxidant compounds. Cultivar choice was most significant for callus production and the synthesis of health-promoting metabolites. The best combination, with respect to the induction efficacy and antioxidant properties measured as a synthetic value by Multidimensional Comparative Analysis (MCA), was found in the callus of cultivar &lsquo:Flacoro&rsquo:, cultivated without MgO fertilization and on a medium with kinetin (KIN) and 1-naphthaleneacetic acid (NAA) (MCA-value 0.465). The worst performance was found for cultivar &lsquo:Yello Mello&rsquo:, independent of the applied phythormones (averaged MCA-value 0.839) and for the cultivar &lsquo:Flacoro&rsquo: fertilized with MgO and independent of growth hormones (averaged MCA-value 0.810).
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