Interaction between Growth Regulators Controls In Vitro Shoot Multiplication in Paulownia and Selection of NaCl-Tolerant Variants
2022
Jehan Salem | Ahmed Hassanein | Deiaa A. El-Wakil | Naglaa Loutfy
The interaction between cytokinin, auxin and GA controlled in vitro shoot multiplication in paulownia was influenced by a medium water potential (&Psi:) modulation, where it was modulated using different textures or strengths of MS medium, media of different types (MS, WPM, SH and B5) or NaCl incorporation. The interaction between 2 mg/L BAP and 0.1 mg/L NAA expressed the highest shoot number on each media type, but it was better with media of lower water potential (MS and WPM), and MS medium was the best. &Psi: of full-strength semisolid MS medium expressed the highest shoot multiplication. The opposite was detected when &Psi: of MS medium was changed using half- or double-strength MS. &Psi: of full-strength MS medium in semisolid form resulted in a valuable interaction between 2 mg/L BAP, 0.1 mg/L NAA and 0.1 mg/L GA, leading to efficient shoot formation, and it was associated with an increase in internode length and decrease in stem diameter, which facilitated obtaining synseeds with a high ability to convert. High genetic variation was recorded under long-term culture (14 subcultures). Polymorphism using the ISSR technique was higher than that of RAPD. A further increase in polymorphism was detected when NaCl was used, where five salt-tolerant lines were selected. Some salt-tolerant-selected lines showed one or more amplification products of a specific molecular weight that did not appear in the control. For example, with OPA-07 and OPG-02 RAPD primers, all the salt-tolerant-selected lines showed the appearance of amplification fragments (610 bp and 300 bp, respectively) that were not detected in control.
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