Enterococcus casseliflavus KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells
2022
Shohei Satomi | Daichi Kokubu | Takuro Inoue | Masaya Sugiyama | Masashi Mizokami | Shigenori Suzuki | Kazumoto Murata
The association between lactic acid bacteria (LAB) and their immunostimulatory effects has attracted considerable attention: however, it remains unclear whether LAB can induce interferon-lambdas (IFN-&lambda:s) in human epithelial cells under conditions that do not mimic infection. In this study, we first employed a reporter assay to screen for a potential strain capable of inducing IFN-&lambda:3 among 135 LAB strains derived from traditional Japanese pickles. Next, we assessed the strain&rsquo:s ability to induce the expression of IFN-&lambda: genes and interferon-stimulated genes (ISGs), and to produce IFN-&lambda:s. As a result, we screened and isolated Enterococcus casseliflavus KB1733 (KB1733) as a potential strain capable of inducing IFN-&lambda:3 expression. Furthermore, we clarified that KB1733 induced the expression of IFN-&lambda: genes and ISGs related to antiviral functions, and that KB1733 induced IFN-&lambda:1 and -&lambda:3 expression in a dose-dependent manner up to 10 &mu:g/mL. In addition, KB1733 significantly increased IFN-&lambda:1 production compared to Enterococcus casseliflavus JCM8723T, which belongs to the same genera and species as KB1733. In conclusion, we isolated a unique LAB strain from traditional Japanese pickles that is capable of stimulating IFN-&lambda: production, although further study is needed to investigate how KB1733 protects against viruses in mice and humans.
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