Intestinal Ecology Changes in Diarrheic Père David’s Deer Revealed by Gut Microbiota and Fecal Metabolites Analysis
2022
Junai Zhen | Xueli Yuan | Liping Tao | Huidan Zhang | Yijun Ren | Shengbin Xie | Libo Wang | Hua Shen | Yuqing Chen
Diarrhea is one of the most common diseases affecting the health of Pè:re David&rsquo:s deer (Elaphurus davidianus). It is believed that an imbalanced intestinal ecology contributes to the etiology of the condition. However, little is known about how the intestinal ecology changes in these diarrheic animals. In this study, 16S rRNA gene sequencing and ultra-high performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS) were used to investigate the gut microbiota and fecal metabolites in five Pè:re David&rsquo:s deer with diarrhea. The results showed that when compared with healthy individuals, considerable changes in the gut microbiome were observed in diarrheic animals, including a significant reduction in microbial diversity and gut microbiota composition alterations. Furthermore, the profiles of numerous fecal metabolites were altered in diarrheic individuals, showing large-scale metabolite dysregulation. Among metabolites, acylcarnitines, lysophosphatidylcholine, bile acids, and oxidized lipids were elevated significantly. Constantly, several metabolic pathways were significantly altered. Interestingly, predicted metabolic pathways based on 16S rRNA gene sequence and differential metabolite analysis showed that lipid metabolism, cofactor, and vitamin metabolism were altered in sick animals, indicating microbiota-host crosstalk in these deer. When combined, the results provide the first comprehensive description of an intestinal microbiome and metabolic imbalance in diarrheic Pè:re David&rsquo:s deer, which advances our understanding and potential future treatment of diarrheic animals.
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