Smartphone-Based Electrochemical Biosensor for On-Site Nutritional Quality Assessment of Coffee Blends
2023
Cristine D’Agostino | Claudia Chillocci | Francesca Polli | Luca Surace | Federica Simonetti | Marco Agostini | Sergio Brutti | Franco Mazzei | Gabriele Favero | Rosaceleste Zumpano
This work aimed to develop an easy-to-use smartphone-based electrochemical biosensor to quickly assess a coffee blend&rsquo:s total polyphenols (Phs) content at the industrial and individual levels. The device is based on a commercial carbon-based screen-printed electrode (SPE) modified with multi-walled carbon nanotubes (CNTs) and gold nanoparticles (GNPs). At the same time, the biological recognition element, Laccase from Trametes :versicolor, TvLac, was immobilized on the sensor surface by using glutaraldehyde (GA) as a cross-linking agent. The platform was electrochemically characterized to ascertain the influence of the SPE surface modification on its performance. The working electrode (WE) surface morphology characterization was obtained by scanning electron microscopy (SEM) and Fourier-transform infrared (FT-IR) imaging. All the measurements were carried out with a micro-potentiostat, the Sensit Smart by PalmSens, connected to a smartphone. The developed biosensor provided a sensitivity of 0.12 &mu:A/&mu:M, a linear response ranging from 5 to 70 &mu:M, and a lower detection limit (LOD) of 2.99 &mu:M. Afterward, the biosensor was tested for quantifying the total Phs content in coffee blends, evaluating the influence of both the variety and the roasting degree. The smartphone-based electrochemical biosensor&rsquo:s performance was validated through the Folin&ndash:Ciocâ:lteu standard method.
显示更多 [+] 显示较少 [-]