Hydrogel-Containing Biogenic Silver Nanoparticles: Antibacterial Action, Evaluation of Wound Healing, and Bioaccumulation in Wistar Rats
2023
Sara Scandorieiro | Angela Hitomi Kimura | Larissa Ciappina de Camargo | Marcelly Chue Gonçalves | João Vinícius Honório da Silva | Wagner Ezequiel Risso | Fábio Goulart de Andrade | Cássia Thaïs Bussamra Vieira Zaia | Audrey Alesandra Stinghen Garcia Lonni | Claudia Bueno dos Reis Martinez | Nelson Durán | Gerson Nakazato | Renata Katsuko Takayama Kobayashi
Wound infections are feared complications due to their potential to increase healthcare costs and cause mortality since multidrug-resistant bacteria reduce treatment options. This study reports the development of a carbomer hydrogel containing biogenic silver nanoparticles (bioAgNPs) and its effectiveness in wound treatment. This hydrogel showed in vitro bactericidal activity after 2 h, according to the time&ndash:kill assay. It also reduced bacterial contamination in rat wounds without impairing their healing since the hydrogel hydrophilic groups provided hydration for the injured skin. The high number of inflammatory cells in the first days of the skin lesion and the greater degree of neovascularization one week after wound onset showed that the healing process occurred normally. Furthermore, the hydrogel-containing bioAgNPs did not cause toxic silver accumulation in the organs and blood of the rats. This study developed a bioAgNP hydrogel for the treatment of wounds: it has a potent antimicrobial action without interfering with cicatrization or causing silver bioaccumulation. This formulation is effective against bacteria that commonly cause wound infections, such as Pseudomonas aeruginosa and Staphylococcus aureus, and for which new antimicrobials are urgently needed, according to the World Health Organization&rsquo:s warning.
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