Gα Solicits OsNYC4 and GW2-WG1-OsbZIP47 Modules to Regulate Grain Size in Rice (Oryza sativa L.)
2024
Shiwei Ma | Yiqiong Sun | Xuan Chen | Jiayi Guo | Shuhong Wu | Guofeng Wu | Guanpeng Huang | Manegdebwaoga Arthur Fabrice Kabore | Samuel Tareke Woldegiorgis | Yufang Ai | Lina Zhang | Wei Liu | Huaqin He
Grain size is one of the critical factors determining rice yield. Previous studies have found the grain-size-regulating function of G&alpha: in rice. However, the regulatory mechanism underlying the development of rice grain mediated by G&alpha: is still unclear. To reveal the functional mechanism of G&alpha: in grain size regulation, a mutant of G&alpha: (G&alpha:-Cas9) was firstly constructed through a CRISPR/Cas9 strategy and was then grown in a greenhouse and field. The results showed that the seed length, plant height, 1000-grain weight, and spike length were significantly decreased in G&alpha:-Cas9 compared to wild-type (WT) Pi-4b. During the grain filling stage, the increase in the grain dry weight of Pi-4b occurred earlier than that of G&alpha:-Cas9. The total starch content and amylose content of matured grains of Pi-4b were higher than those of G&alpha:-Cas9. Secondly, transcriptome sequencing analysis of G&alpha:-Cas9 and Pi-4b during grain filling was performed to elucidate the functional pathways regulated by G&alpha:. In total, 2867 and 4534 differentially expressed genes (DEGs) were discovered at 5 DAF and 10 DAF, and the starch and sucrose metabolism pathway enriched by DEGs was involved in grain size regulation mediated by G&alpha:. G&alpha: regulated the expression of starch-synthesis-related genes during grain filling, and the G&alpha: protein interacted with OsNYC4 to trigger the sugar signaling pathway to promote starch accumulation in grain. Additionally, the GW2-WG1-OsbZIP47 pathway was switched off by G&alpha: to relieve the inhibition of rice grain development. In this study, the results should provide new insights into the G protein signal transduction pathway.
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