Bioluminescence Inhibition Bioassay for Estimation of Snow Cover in Urbanised Areas within Boreal Forests of Krasnoyarsk City
2024
Anastasia A. Rimashevskaya | Elena Y. Muchkina | Oleg S. Sutormin | Dmitry E. Chuyashenko | Arsen R. Gareev | Svetlana A. Tikhnenko | Nadezhda V. Rimatskaya | Valentina A. Kratasyuk
It has been proposed that the level of air pollution in a city should be estimated based on the accumulation of pollutants in the snow cover of urban forests. This study presents a bioluminescence method for estimating the extent of snow cover pollution in the urbanised areas of boreal forests in Krasnoyarsk city. A bioluminescent assay involving NAD(P)H:FMN oxidoreductase (Red) and luciferase with luminous bacteria (BLuc) was employed to measure the concentrations of six heavy metals (As, Cd, Zn, Co, Hg, and Pb) in the snow cover. The tested snow samples demonstrated a correlation between the reduced activity of the enzyme system and variations in Cd concentration. Furthermore, the research indicated that the period of unfavourable meteorological conditions in Krasnoyarsk city resulted in a notable decline in the activity of the BLuc&ndash:Red enzyme system, which may be associated with elevated air pollution levels. This study underscores the potential of the bioluminescence method for monitoring environmental pollution in urban forested areas.
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