Triglochin maritima Extracts Exert Anti-Melanogenic Properties via the CREB/MAPK Pathway in B16F10 Cells
2024
Won-Hwi Lee | Yuna Ha | Jeong-In Park | Won Bae Joh | Mira Park | Jang Kyun Kim | Hee-Kyung Jeon | Youn-Jung Kim
Triglochin maritima, a salt-tolerant plant, has demonstrated antioxidant effects, the ability to prevent prostate enlargement, antifungal properties, and skin moisturizing benefits. This study aimed to explore the anti-melanogenic potential of the 70% ethanol extract of T. maritima (TME) along with its ethyl acetate (TME-EA) and water (TME-A) fractions. TME (10&ndash:200 µ:g/mL), TME-EA (1&ndash:15 µ:g/mL), and TME-A (100&ndash:1000 µ:g/mL) were prepared and applied to B16F10 cells with or without &alpha:-MSH for 72 h. MTT assays were used to assess cytotoxicity, and anti-melanogenesis activity was determined by measuring melanin content, conducting a tyrosinase activity assay, and evaluating the expression of melanogenesis-related genes and proteins via RT-PCR and Western blotting. HPLC-PDA was used to analyze TME and TME-EA. The IC20 cytotoxicity values of TME, TME-A, and TME-EA without &alpha:-MSH, were 198.426 &mu:g/mL, 1000 &mu:g/mL, and 18.403 &mu:g/mL, respectively. TME and TME-EA significantly decreased melanin and tyrosinase activity in &alpha:-MSH-stimulated B16F10 cells, with TME-EA showing comparable effects to arbutin, while TME-A showed no influence. TME-EA down-regulated melanogenesis genes (Tyr, Trp1, Dct, Mitf, Mc1r) and reduced CREB, p-38, and JNK phosphorylation while increasing ERK phosphorylation, suggesting the CREB/MAPK pathway&rsquo:s role in the anti-melanogenic effect. Luteolin was identified as a potential active ingredient. TME-EA may serve as an effective cosmeceutical for hyperpigmentation improvement due to its anti-melanogenic properties.
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