GFAPβ and GFAPδ Isoforms Expression in Mesenchymal Stem Cells, MSCs Differentiated Towards Schwann-like, and Olfactory Ensheathing Cells
2025
Nidia Jannette Carrillo González | Gabriela Stefania Reyes Gutierrez | Tania Campos-Ordoñez | Rubén D. Castro-Torres | Carlos Beas Zárate | Graciela Gudiño-Cabrera
Olfactory ensheathing cells (OECs) and mesenchymal stem cells (MSCs) differentiated towards Schwann-like have plasticity properties. These cells express the Glial fibrillary acidic protein (GFAP), a type of cytoskeletal protein that significantly regulates many cellular functions, including those that promote cellular plasticity needed for regeneration. However, the expression of GFAP isoforms (&alpha:, &beta:, and &delta:) in these cells has not been characterized. We evaluated GFAP isoforms (&alpha:, &beta:, and &delta:) expression by Polymerase Chain Reaction (PCR) assay in three conditions: (1) OECs, (2) cells exposed to OECs-conditioned medium and differentiated to Schwann-like cells (dBM-MSCs), and (3) MSC cell culture from rat bone marrow undifferentiated (uBM-MSCs). First, the characterization phenotyping was verified by morphology and immunocytochemistry, using p75, CD90, and GFAP antibodies. Then, we found the expression of GFAP isoforms (&alpha:, &beta:, and &delta:) in the three conditions: the expression of the GFAP&alpha: (10.95%AUC) and GFAP&beta: (9.17%AUC) isoforms was predominantly in OECs, followed by dBM-MSCs (&alpha:: 3.99%AUC, &beta:: 5.66%AUC) and uBM-MSCs (&alpha:: 2.47%AUC, &beta:: 2.97%AUC). GFAP&delta: isoform has a similar expression in the three groups (OEC: 9.21%AUC, dBM-MSCs: 11.10%AUC, uBM-MSCs: 9.21%AUC). These findings suggest that expression of different GFAP&delta: and GFAP&beta: isoforms may regulate cellular plasticity properties, potentially contributing to tissue remodeling processes by OECs, dBM-MSCs, and uBM-MSCs.
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