Production of an Extract with β-1,4-Xylanase Activity by Fusarium oxysporum f. sp. melonis on a Sonicated Brewer’s Spent Grain Substrate
2025
Irma A. Arreola-Cruz | Rosalba Troncoso-Rojas | Francisco Vásquez-Lara | Nina G. Heredia-Sandoval | Alma R. Islas-Rubio
The Fusarium oxysporum species commonly found in soil include plant and human pathogens, and nonpathogenic species. F. oxysporum grown on lignocellulosic substrates under submerged conditions produces an extracellular enzyme profile with hemicellulolytic and cellulolytic activities. Our aim was to produce an extract of Fusarium oxysporum f. sp. melonis with &beta:-1,4-xylanase activity after fermentation on a Brewers&rsquo: spent grain (BSG)-containing substrate. We prepared the BSG substrate, with or without sonication, for the submerged fermentation of Fusarium oxysporum previously isolated from local soil and preserved at 4 °:C. First, an enriched inoculum was prepared, and later, the production of &beta:-1,4-xylanase using the BSG substrates was monitored for up to 6 or 10 days in the enriched inoculum or in the enzyme extract, respectively. An activity of &beta:-1,4-xylanase 12.0 U/mL (day 3) was obtained in the enriched inoculum with the untreated BSG, remaining constant for 3 days. A significant increase in the activity of this enzyme was observed (day 6), especially in the extract obtained using the sonicated BSG substrate (39 U/mL). Applying ultrasound to the BSG before its use in a submerged fermentation with Fusarium oxysporum f. sp. melonis could be an alternative for producing &beta:-1,4-xylanase.
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