Synergistic Effects of Microbial Biostimulants and Calcium in Alleviating Drought Stress in Oilseed Rape
2025
Virgilija Gavelienė | Rima Mockevičiūtė | Elžbieta Jankovska-Bortkevič | Vaidevutis Šveikauskas | Mariam Zareyan | Tautvydas Žalnierius | Jurga Jankauskienė | Sigita Jurkonienė
The study aimed to examine the changes in winter oilseed rape (Brassica napus L.) under simulated prolonged drought and to assess the effects of a microbial biostimulant ProbioHumus and calcium, individually and in combination, in order to improve the plant&rsquo:s drought resistance and to identify the biochemical processes occurring in the plant tissues. The oilseed rape cv. &lsquo:Visby&rsquo: was grown under controlled laboratory conditions. CaCO3 (hereafter, Ca) (3.71 g) was added to the soil of one pot at 70 g m&minus:2. Seedlings at the 3&ndash:4 leaf stage were sprayed with ProbioHumus 2 mL 100 mL&minus:1 and exposed to drought for 8 days to achieve a high water deficit. Irrigation was then resumed, and recovery was assessed after 4 days. The data showed that the microbial biostimulant alleviated the physiological and biochemical response of oilseed rape to drought stress. ProbioHumus + Ca reduced plant wilting by increasing leaf relative water content (RWC) by 87% and induced drought tolerance by increasing endogenous proline content 4-fold, increasing photosynthetic pigment content in leaves by 10&ndash:28%, reducing H2O2 by 53% and malondialdehyde (MDA) by 45%, and stimulating stomata opening (by 2-fold on the upper and 1.4-fold in the lower leaf surface), vs. drought control. The most effective measure to increase plant survival and/or resume growth after drought was the application of a microbial biostimulant with additional calcium to the soil. The practical implications of this research point to the potential benefits of applying these ecological measures under field conditions.
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