Influence of Light Intensity and Nutrient Concentration on Soybean (Glycine max (L.) Merr.) Seedling Growth

Light and nutrient availability are critical factors of plant growth and development, particularly at the early stages, where they significantly influence the establishment and survival of young seedlings. The morphological parameters and the biomass accumulation of soybean were measured in a hydroponic vertical farm in the first 14 days of seedling growth in two successive experiments under two types of lighting environments and at three nutrient concentration levels. The lighting conditions were set by two parallel variable-spectrum linear luminaires positioned above the lower and upper edges of the cultivation trays. In the first lighting environment, seedlings were exposed to a constant photosynthetic photon flux density (PPFD) with red and blue photon irradiance ratio (R/B) varying in broad range from the lower to the upper end of the cultivation trays. In the second environment, the spatial R/B distribution was uniform, and the PPFD varied between two maxima at the edges and a minimum in the middle of the trays. The R/B ratio within the 0.6&ndash:6 interval had little or no effect on plant development. We report the dependence of growth traits as a function of PPFD in the range of 30&ndash:290 &micro:mol m&minus:2 s&minus:1 in full-strength, half-strength, and blank nutrient solutions. The light response for shoot height and the first internode length was mainly influenced by blue light. We observed a rapid decline in growth between 6&ndash:20 &micro:mol m&minus:2 s&minus:1 blue photon irradiance. The shoot height and first internode length did not change significantly at higher blue light intensities. The lengths of the first internode and the root dry mass did depend on the nutrient solution strength. All other growth traits, including stem diameter, leaf size, shoot mass, root mass, and SPAD readings, showed a linear correlation with PPFD and electrical conductivity. The leaf mass and root mass ratios indicated that soybeans adopt a nutrient search strategy by giving preference for root growth while increasing shoot height at the expense of the shoot diameter in conditions of low nutrient availability and low light intensity. The functional relationships determined in the experiments provide valuable inputs to plant growth models. The methodology we employed could also be used to study other plant species and to investigate the interactive effects of specific nutrients and lighting conditions.