In Vitro Embryo Culture Impacts Heart Mitochondria in Male Adolescent Sheep
2025
Reza Amanollahi | Stacey L. Holman | Ashley S. Meakin | Monalisa Padhee | Kimberley J. Botting-Lawford | Song Zhang | Severence M. MacLaughlin | David O. Kleemann | Simon K. Walker | Jennifer M. Kelly | Skye R. Rudiger | I. Caroline McMillen | Michael D. Wiese | Mitchell C. Lock | Janna L. Morrison
Assisted reproductive technology (ART)such as in vitro embryo culture (IVC), is widely used in human infertility treatments: however, its long-term effects on the cardiac health of offspring remain unclear. This study aimed to determine whether the effects of IVC on cardiac metabolism and associated signaling pathways persist after birth into adolescence. Embryos were either transferred to an intermediate ewe (ET) or cultured in vitro in the absence (IVC) or presence of human serum (IVCHS) with methionine supplementation (IVCHS+M) for 6 days after mating. Naturally mated (NM) ewes were used as controls. Protein expression and hormone concentrations in the left ventricle (LV) were analyzed using Western blot and LC-MS/MS analyses, respectively. IVC was associated with sex-specific alterations in cardiac mitochondria, with males exhibiting reduced mitochondrial abundance. Cardiac protein expression of oxidative phosphorylation (OXPHOS) complexes 1 and 4 was reduced by IVC. Additionally, IVC reduced protein expression of PDK-4 and Mn-SOD in the IVCHS+M group, which may impact energy efficiency and defense against oxidative stress. These changes may predispose IVC offspring to cardiac oxidative stress and mitochondrial dysfunction, particularly in males. This study provides insights into the sex-dependent effects of IVC on cardiac health, emphasizing the importance of evaluating long-term cardiovascular risks associated with IVC protocols.
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