Biological Traits and Isoenzymatic Characterization of Triplophysa strauchii from the Turks River

The intensification of human activities in recent years has led to significant overexploitation of Triplophysa strauchii populations, resulting in a decline in the species&rsquo: natural stocks. This underscores the need for research and development initiatives aimed at supporting the recovery and sustainable management of the species. Therefore, this study investigated its biological traits and isozyme characteristics in detail. First, throutigations of fish ecology, the age and growth patterns of T. strauchii were examined. The results revealed that the length of the otoliths was greater than the width and that the intermajor groove was indistinct. The age range of the fish was 0&ndash:4 years. A correlation between body length and weight revealed that T. strauchii exhibited isometric growth patterns. In terms of growth parameters, the inflection point in age for T. strauchii was ti = 3.23. Additionally, to analyze the enzymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), and esterase (EST) in ten tissues of T. strauchii (liver, muscle, heart, gills, eye, brain, fins, kidneys, gonads, and intestines), vertical plate electrophoresis was performed via polyacrylamide gels. The results of isoenzyme analysis revealed that the LDHA (lactate dehydrogenase A subunit) gene was predominant in all tissues. A maximum of two s-MDH enzyme bands with three m-MDH enzyme bands were detected, with a classic enzyme profile and no gene mutation. The EST enzyme was highly expressed in the liver and kidney and was less polymorphic. In general, T. strauchii exhibited a spindle-like body shape and isometric growth patterns in the Turks River. It exhibited a narrow age range, strong adaptability, and stable genetic traits. This species has high development potential, utilization value and ecological significance.